identification of live toxoplasma gondii by the nasba method in rat

نویسندگان

roghaye noruzi

abdolhossein dalimi

mehdi forouzandeh

fatemeh ghaffarifar

چکیده

objective: toxoplasmosis is a worldwide disease, for which different detection methods have been used. the nucleic acid sequence-based amplification (nasba) method is shown to be highly efficient for diagnosis of live microorganisms. the present research evaluates the molecular isothermal method of nasba to identify live toxoplasma gondii (t. gondii) in rat. methods: tachyzoites of t. gondii were inoculated in the peritoneal cavities of mice (mus musculus) and their rna was extracted. the nasba method was then used to amplify the tachyzoite b1 rrna gene. next, we examined blood samples from 30 experimentally infected case and control rats (rattus norvegicus) by nasba. finally, the resultant band was investigated on an agarose gel. results: the b1 genes extracted from both the tachyzoites and blood samples were successfully amplified by the nasba method. this amplified gene yielded an amplicon of approximately 116 bp on gel agarose. conclusion: nasba is highly efficient for the identification of live t. gondii. this method can be applied for early diagnosis of active toxoplasmosis in both newborns and immunocompromised individuals.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Molecular Identification of Toxoplasma gondii in the Native Slaughtered Cattle of Tehran Province, Iran

Background: Toxoplasmosis, caused by Toxoplasma gondii, is a common parasitic disease, affecting almost one-third of the world’s population. It is transmitted by ingestion of food or water contaminated with oocysts excreted by cats and the consumption of raw or undercooked meat from ruminants. This study aimed at molecular characterization of T. gondii in native cattle from West of Tehran, Iran...

متن کامل

study of cohesive devices in the textbook of english for the students of apsychology by rastegarpour

this study investigates the cohesive devices used in the textbook of english for the students of psychology. the research questions and hypotheses in the present study are based on what frequency and distribution of grammatical and lexical cohesive devices are. then, to answer the questions all grammatical and lexical cohesive devices in reading comprehension passages from 6 units of 21units th...

Prevalence of Toxoplasma gondii in the Slaughterhouse Workers of Tabriz

ABSTRACT Since Toxoplasma is an important infection can be found in human and animal. For serological evaluation of toxoplasma, present study conducted on serum sampels of 144 slaughterhouse workers as case group and 67. Serum sampels of healthy blood donors as control group. All of serum sampels were examined by indirect immunoflouroscence antibody (IFA) method. Difference of antibody titer...

متن کامل

Vaccine design based on Live attenuated cells of Toxoplasma gondii: a review

Toxoplasma gondii is well- known as a common parasite with high proliferative activity in the nucleated cells. This parasite has infected nearly one third of the people worldwide. Infection can result in chorioretinitis in immunocompetent hosts, encephalitis in HIV/AIDS positive patients and abortion and neonatal loss in congenital toxoplasmosis. Development of vaccines for toxoplasmosis infect...

متن کامل

Polytenosynovitis caused by Toxoplasma gondii.

Two cases are reported of polytenosynovitis involving the wrists and ankles and caused by toxoplasmosis, together with the investigations that led to the diagnosis. Medical and surgical treatment of the first patient and medical treatment only of the second patient gave satisfactory results.

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
modares journal of medical sciences: pathobiology

ناشر: tarbiat modares university

ISSN 1562-9554

دوره 15

شماره 1 2012

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023